Rickettsia pada Pinjal Tikus (Xenopsylla Cheopis) di Daerah Pelabuhan Semarang, Kupang dan Maumere

Arum Sih Joharina, Arief Mulyono, Tika Fiona Sari, Esti Rahardianingtyas, Dimas Bagus Wicaksonoputro, Noer Endah Pracoyo, Ristiyanto Ristiyanto

Abstract


Abstract

The genus of Rickettsia is gram negative bacteria causing rickettsioses and involve mammal hosts and arthropod vectors in their life cycle (lices, mites, ticks, and fleas). Rats were one of rickettsial hosts, and fleas were rat ectoparasites that involve in the transmision from bacteria into humans. Unspecific clinical manifestation and difficulties of laboratory diagnoses caused the information about rickettsioses in humans were still limited. The aim of this study was to detect Rickettsia spp on rat fleas. Rat flea specimens were collected from three seaports of Semarang, Kupang and Maumere. Specimens were analyzed using PCR method by gltA amplification (primer 877F and 1258R). Confirmation of rickettsia species was conducted by sequencing. The results showed percentages of rickettsial infections on rat fleas for Semarang, Kupang, and Maumere were 19%, 61%, and 44%, respectively. Seven samples from eighteen samples sequences confirmed as Rickettsia typhi and the other 11 samples were Bartonella sp. This study was provided additional information about the presence of Rickettsia in 3 seaport in Indonesia and could be initiating rickettsioses surveilans in the regions.

Keywords: Rickettsia spp., Xenopsylla cheopis, rat, molecular, Indonesia

Abstrak

Rickettsia merupakan bakteri pathogen penyebab berbagai rickettsiosis yang siklus hidupnya melibatkan reservoir mamalia dan vektor artropoda (kutu, tungau, caplak, dan pinjal). Tikus merupakan salah satu reservoir bakteri Rickettsia dan pinjal merupakan ektoparasit tikus yang berperan menularkan bakteri kepada manusia. Gejala klinis tidak spesifik dan diagnosis laboratorium yang sulit menyebabkan informasi mengenai rickettsiosis pada manusia masih sangat terbatas. Tujuan penelitian adalah mendeteksi Rickettsia spp. pada pinjal. Sampel pinjal tikus diambil dari tiga daerah pelabuhan yaitu Semarang, Kupang, dan Maumere. Sampel dianalisa menggunakan metode PCR dengan amplifikasi gen gltA (primer 877F dan 1258R). Sekuensing dilakukan untuk mengkonfirmasi spesies Rickettsia. Hasil penelitian menunjukkan bahwa persentase Rickettsia spp. pada pinjal tikus di Semarang 19%, Kupang 61%, dan Maumere 44%. Tujuh sampel dari 18 sampel yang disekuensing terkonfirmasi sebagai R.typhi dan 11 sampel sisanya merupakan Bartonella sp. Penelitian ini memberikan informasi tambahan tentang keberadaan Rickettsia di beberapa kota pelabuhan di Indonesia dan dapat dijadikan sebagai dasar surveilans rickettsiosis di Indonesia.

Kata kunci: Rickettsia spp., Xenopsylla cheopis, tikus, molekuler, Indonesia


Keywords


Rickettsia spp., Xenopsylla cheopis, rat, molecular, Indonesia

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