Diversitas Genetik Anopheles balabacensis, Baisas di Berbagai Daerah Indonesia Berdasarkan Sekuen Gen ITS 2 DNA Ribosom

Widiarti Widiarti, Triwibowo Ambar Garjito, Umi Widyastuti



Malaria control is remain a challenge although various attempts have been conducted. One of the issues in controlling the vectors is the presence of species complex. The species complex is an example of genetic diversity. Anopheles balabacensis, Baisas reported as complex species in various countries, but has not been widely reported in Indonesia. In order to enhance malaria control, it is important to understand the vectors and its bioecology. The aim of the study were a). to identify An. balabacensis, Baisas suspected as species complex based on ribosomal DNA the second internal transcribed spacer (ITS2) gene sequences, b). to understand the genetic diversity of An. balabacensis, Baisas collected from endemic and non endemic regions distincted by geographical distance, c). to understand the genetic relationships (taxonomi distance) among An. balabacensis, Baisas from difference regions in Indonesia through reconstructing the phylogenetic trees. The results showed that An. balabacensis, Baisas in Indonesia is identified as sympatric and allopatrik complex species. There were differences which was far enough in the genetic relationships among An. balabacensis populations collected from Pusuk Lestari in the area of Meninting Health Center, West Lombok, NTB. This differences were identified as sympatric complex. In addition, base on the relationship among An. leucosphyrus group, An balabacensis, Baisas collected from Berjoko Nunukan Regency showed that the species quite far compare to An. balabacensis, Baisas originally from Central Java and Lombok NTB.

Keywords : An. balabacensis, genetic variation, the second Internal Transcribed Spacer (ITS2).


Penanggulangan malaria masih banyak menemui kendala walaupun berbagai upaya telah dilakukan. Salah satu kendala yang menyulitkan dalam pengendalian vektor adalah adanya spesies kompleks pada populasi nyamuk vektor. Spesies kompleks merupakan contoh diversitas genetik. Anopheles balabacensis dilaporkan sebagai spesies kompleks di berbagai negara, akan tetapi belum banyak dilaporkan di Indonesia. Penanggulangan malaria agar lebih efektif perlu adanya perbaikan dan pendekatan strategi dalam pengendalian vektor, termasuk sangat diperlukan adanya pemahaman terhadap spesies dan bioekologinya. Tujuan penelitian adalah untuk : a). Mengidentifikasi secara molekuler nyamuk An. balabacensis yang dicurigai sebagai spesies kompleks berdasarkan sekuen ITS2 DNA ribosom, b). Mengetahui diversitas genetik nyamuk An. balabacensis dari daerah endemis dan non endemis dengan jarak geografis yang berbeda, c). Mengetahui kekerabatan genetik (jarak taksonomi) nyamuk An. balabacensis dari berbagai daerah di Indonesia dengan merekonstruksi pohon filogenetik. Hasil penelitian menunjukkan bahwa An. balabacensis di Indonesia merupakan spesies kompleks simpatrik dan allopatrik. Ada perbedaan kekerabatan genetik
yang cukup jauh diantara populasi An. balabacensis di Pusuk Lestari, wilayah Puskesmas Meninting, Lombok Barat, NTB yang merupakan simpatrik kompleks. Berdasarkan hubungan
kekerabatan An. leucosphyrus group, An. balabacensis dari Berjoko, Kabupaten Nunukan menunjukkan kecenderungan terpisah cukup jauh dibandingkan dengan An. balabacensis
kompleks lainnya yang berasal dari Jawa Tengah dan Lombok, NTB.

Kata kunci : An. balabacensis, variasi genetik, ITS2 DNA ribosom


An. balabacensis, genetic variation, the second Internal Transcribed Spacer (ITS2)


Kementerian Kesehatan RI. Epidemiologi Malaria di Indonesia. Buletin Jendela Data dan Informasi Kesehatan. RI. Triwulan I; 2011

Harijanto, PN. Malaria, epidemiologi, patogenesis, manifestasi klinis dan penanganan. Jakarta:EGC;1999.

Dinkes Provinsi Jawa Tengah. Gebrak Malaria. 2003.

Coluzzi M, V. Petrarca dan MA. Dideco. Chromosomal inversion intergradation and incipient speciation in An. gambiae. Bull. Zool. 1985; 52: 45-63

Service, MW and H. Townson. The Anopheles vector. In Warrel D and HM. Gilles. Essential Malariology. London: Hodder Headline Group London; 2004

Dharmawan R. Metoda identifikasi spesies kembar nyamuk Anopheles. Solo: Sebelas Maret Univ. Press;1993.

WHO. Anopheline species complexes in South and South-east Asia. SEARO Technical Publication. 2007; (57)

Barodji, Boesri H, Boewono DT dan Sumardi. Bionomik vektor malaria di daerah endemis malaria Kecamatan Kokap Kabupaten. Kulonprogo, DIY. 2001.

Boewono DT dan Ristiyanto. Studi bioekologi vektor malaria di Kecamatan Srumbung, Kabupaten Magelang. Buletin Penelitian Kesehatan. 2005; 33(2):

Maekawa Y, T. Suhanara, YP. Dachlan, S. Yotoranoto, S. Basuki, H. Uemura, H. Kanbara, dan M. Takagi. First record of An. balabacencis from western Sumbawa Island, Indonesia. J. Am. Mosq. Control. Assoc. 2009;25(2):203-205.

Tsun HY. A review of literature on An. balabacencis balabacencis. WHO/MAL/83.999. WHO/VBC/83.873. 1983;

Hii, JLK. Evidence for the Existence of Genetik Variability in the Tendency of An. balabacensis to Rest in Houses and to Bite Man. SeameoTropmed Technical Meeting: Mosquito Vectors of Malaria in Southeast Asia. Bangkok, Thailand. 1985;

Jia-Siang Sum, Wenn-Chyau Lee, Amirah Amir, Kamil A Braima, John Jeffery, Noraishah M Abdul-Aziz, Mun-Yik Fong and Yee-Ling Lau. Phylogenetic study of six species of Anopheles mosquitoes in Peninsular Malaysia based on inter-transcribed spacer region 2 (ITS2) of ribosomal DNA. Parasites &Vectors. 2014;7(309):1-8.

Marsden C, D., Lee, Y., Kreppel, K. Weakley, A., Cornel A., Ferguson, H.M., Eskin, E., et.al. G.C. Diversity, differentiation and linkage disequilibrium: prospects for association mapping in the malaria vector, Anopheles arabiensis. Genetics Society of America; 2013

WHO. Malaria entomology and vector control. Leaner’s guide. WHO HIV/AIDS, Tuberculosis and Malaria, Rollback Malaria. Trial Ed. WHO/ CDS/CPE/SMT/2002. 2003; 18 Rev.1. Part 1.

O’Connor, CT and A. Soepanto. Kunci bergambar untuk Anopheles betina di Indonesia. Dit. Jen P3M, Depkes RI, 1979. Jakarta: Dit. Jen P3M, Depkes RI;1979.

Dhananjeyan, KJ., R. Paramasivan, SC. Tewari, R. Rajendran, V. Tenmozhi, SVJ. Leo, A. Venkatesh, and BK.Tyagi. Molecular identification of mosquito vectors using genomic DNA isolated from eggshells, larval and pupal exuvium. Trop. Biomed. 2010;27(1): 47-53.

Roche (www.roche-applied-science.com). High pure PCR template preparation kit. Version 20. Roche Diagnostics GmbH, Roche Applied Science, 68298 Manheim, Germany. 2012: 26 p.

Sharma M & S. Chaudhry. Molecular cytogenetics of some Anopheles mosquitoes (Culicidae: Diptera). E. Journal of Biol. 2010; 6(1):13-18

Tamura K, D. Peterson, N. Peterson, G. Stecher, M. Nei, & S. Kumar. 2011. Mega 5.05.

Takano, KT., NTH. Nguyen, BTH. Nguyen, T. Sunahara, M. Yasunami, MD. Nguyen, and M. Takagi. Partial mitochondrial DNA sequences suggest the existence of a cryptic species within the Leucosphyrus group of the genus Anopheles (Diptera: Culicidae), forest malaria vectors, in northern Vietnam. Parasite & Vectors. 2010;3(41): 1-16

Paul, S., A. Chattopadhyay and P. K. Banerjee. Anopheline diversity: morphological and molecular variation of an. subpictus in rural and urban areas of west Bengal. Journal of Entomology and Zoology Studies 2013;1(2):35-40

Laura C.N and E.N.Douglas. Phylogeny of Anopheline (Diptera : Culicidae) species in Southern Africa, based on nuclear and mitochondrial genes. 2015. Journal of Vector

Ecology.2015; 40(1):16-27

Abdelrafie M.M; A.A. Mariam; M.E. Fathi; O.F. Omran; A.E. Dia-Edin. Identification of anopheles species of the funestus group and their role in malaria transmission in Sudan. Journal of

Applied and Industrial Sciences. 2015;3(2):58-62

Manni M; M.G. Ludvik; A.Nidchaya ; T.Gabriella; S.Francesca; P.Somboon; R.G. Carmela; R.M. Anna and G. Gasperi. Molecular markers for analyses of intraspecific genetic diversity in the Asian Tiger Mosquito, Aedes albopictus. Parasites & Vectors. 2015; 8:188.

Mourya DT; R.Kumar; PV. Barde ; MD. Gokhale; and PD. Yadav. Genetic variation in aedes aegypti mosquito population along the west cost of India and their susceptibility to insecticides and dengue virus. 2015. Medical Science Volume : 5 Issue 1.p 378-383.

Walter J. Tabachnick. Nature, nurture and evolution of intra-species variation in mosquito arbovirus transmission competence. Int. J. Environ. Res. Public Health 2013, 10, 249-277

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