PLAQUE ASSAY OF NEWCASTLE DISEASE VIRUS

B. Sardjono

PLAQUE ASSAY OF NEWCASTLE DISEASE VIRUS

Abstract


The Newcastle disease virus (NDV) was isolated from a 3 months-old indigenous chicken (buras or kampung chicken) which showed clinical signs of Newcastle disease (ND). For viral isolation a small part of the spleen and lung were inoculated into 10 days-old embryonated chicken eggs. The physical characteristics of the isolate (A/120) were studied. The hemagglutination of chicken red blood cell showed slow elution, thermostability of hemagglutinin at 56°C was 120 minutes. The vims was able to agglutinate horse erythrocytes but not those of sheep. The biological characteristics on mean death time (MDT) of embryonated chicken egg and plaque morphology on chicken embryo fibroblast (CEF) primary cell cultures were studied. The MDT was 56 hours, the isolate was velogenic NDV. There were three different plaque morphologies on CEF : 2 mm clear plaques, 1 mm clear plaques, and minute clear plaques which were visible only with microscopic examination.


Keywords


Newcastle disease virus (NDV); Plaque Assay

Full Text: PDF

Refbacks

  • There are currently no refbacks.



Buletin Penelitian Kesehatan (Bulletin of Health Research, p-ISSN: 0125-9695. e-ISSN: 2338-3453) is published by Badan Penelitian dan Pengembangan Kesehatan, Ministry of Health of Republic of Indonesia
Main Indexing :

More...
Visitor Number : View BPK Statistics
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.