Ekspresi IFN-Γ oleh Sel T CD4+ dan CD8+ Setelah Stimulasi Antigen Fusi ESAT-6- CFP-10 pada Pasien Tuberkulosis Paru Aktif

Rahmad Yudha Wibowo

Abstract


Abstract
Tuberculosis (TB) remains a major global health problem. T-cells mediated immunity contribute to protection from Mycobacterium tuberculosis infection. CD4+ and CD8+ T-cells secreted interferon gamma (IFN-γ) to stimulate fusion of phagolyzosome and produce free radicals for killing mycobacteria. Early secretory antigenic target 6 kD(ESAT-6) and culture filtrate protein 10(CFP-10) are dominant M. tuberculosis antigens in inducing IFN-γ. This study compared CD4+ and CD8+T-cells expressing IFN-γ with and without ESAT-6- CFP-10 fusion antigen stimulation in active tuberculosis patients. Peripheral blood mononuclear cellsfrom active TB patients were cultured with and without ESAT-6-CFP-10 fusion antigen. CD4+ and CD8+ T-cells expressing IFN-γ were examined using flow cytometry.CD4+ T-cells expressing IFN-γ after antigen stimulation compare to controls(1.51%±1.7 vs 1.23%±0.86; p=0.08),and CD8+ T-cells (1.58%±0,99 vs 1.92%±1.13; p=0.86).CD4+ and CD8+T-cells expressing IFN-γ after antigen stimulation (1.51%±1.7 vs 1.58%±0,99; p=0.94).There were no significant differences of CD4+ and CD8+T-cells expressing IFN-γ after ESAT-6-CFP-10 fusion antigen stimulation in active pulmonary tuberculosis patients.

Key word: CD4+, CD8+, IFN-γ, ESAT-6-CFP-10 fusion antigen

Abstrak
Tuberkulosis masih menjadi masalah kesehatan di dunia. Imunitas yang diperantarai sel T berperan penting dalam perlindungan terhadap infeksi Mycobacterium tuberculosis. SelTCD4+ dan CD8+ mensekresi IFN-γ yang menstimulasi fusi fagolisosom dan pembentukan radikal bebas untuk menghancurkan M. tuberculosis. ESAT-6 dan CFP-10 merupakan antigen M. tuberculosis yang dominan menginduksi IFN-γ. Penelitian membandingkan selT CD4+ dan CD8+ yang mengekspresikan IFN-γ setelah stimulasi antigen fusi ESAT-6-CFP-10 pada TB aktif.Sampel PBMC pasien TB aktif dikultur dengan dan tanpa stimulasi antigen fusi ESAT-6-CFP-10. Persentase sel TCD4+ dan CD8+ yang mengekspresikan IFN-γ diperiksa dengan flow cytometry. Sel TCD4+ yang mengekspresikan IFN-γ setelah stimulasi antigen dibandingkan dengan kontrol (1,51%±1,7 vs 1,23%±0,86; p=0,08) dan sel TCD8+ yang mengekspresikan IFN-γ
(1,58%±0,99 vs 1.92%±1.13; p=0,86). Tidak terdapat perbedaan yang signifikan dalam ekspresi IFN-γ antara sel T CD4+ dan sel T CD8+ setelah stimulasi antigen fusi ESAT6-CFP10 pada pasien TB paru aktif.

Kata kunci: Sel T, CD4+, CD8+, IFN-γ, antigen fusi ESAT-6 dan CFP-10


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