DHF cases dominated by Den-3 serotype in the West Java province

Prasetyowati Prasetyowati, Roy Nusa

DHF cases dominated by Den-3 serotype in the West Java province

Abstract


Abstrak

Latar belakang: Jawa Barat merupakan salah satu propinsi endemis DBD di Indonesia, tetapi data tentang distribusi serotipe di Jawa Barat masih terbatas pada daerah tertentu dengan kurun waktu yang berbeda. Pada tahun 2000- 2002 serotipe Den-2 mendominasi di Kota Bandung. Tujuan dari penelitian ini adalah untuk mengetahui distribusi serotipe virus Dengue di Propinsi Jawa Barat dan serotipe yang mendominasi pada kasus DBD.

Metode: Pengumpulan data dilaksanakan selama bulan Oktober-Desember 2008 di 27 rumah sakit di Propinsi Jawa Barat. Populasi dalam penelitian ini adalah semua penderita yang didiagnosis terinfeksi virus dengue yang dirawat di 27 rumah sakit. Pemilihan sampel secara purposif yaitu penderita yang didiagnosis terinfeksi virus dengue yang bersedia berpartisipasi untuk selanjutnya dilakukan pemeriksaan imunologi. Penderita yang hasil pemeriksaan imunologinya positif diambil serumnya guna pemeriksaan serotipe virus dengue. Pemeriksaan serotipe virus dengue pada sampel serum dilakukan di Pusat Biomedis dan Teknologi Dasar Kesehatan Badan Litbangkes RI dengan menggunakan nested RT-PCR.

Hasil: Dari 513 sampel yang diperiksa, sampel yang dapat teridentifi kasi serotipe virus Dengue adalah sebanyak 180 sampel. Sisanya tidak ditemukan adanya virus dengue dalam serum. Terdapat empat sampel
yang menunjukkan adanya infeksi ganda 2 serotipe virus Dengue. Keempat serotipe ditemukan di Propinsi Jawa Barat. Dari keseluruhan sampel yang positif, Den-3 merupakan serotipe yang paling banyak muncul yaitu sebanyak 47,8%, disusul Den-2 (26,0%), Den-1 (18,6%), Den-4 (5,4%) dan Mix (2,2%). Den-3 ditemukan di semua daerah endemis di Propinsi Jawa Barat.

Kesimpulan: Keempat serotipe virus dengue di temukan Propinsi Jawa Barat yang didominasi serotipe Den-3. (Health Science Indones 2012;1:23-6)

Kata Kunci: serotipe den-3, distribusi serotipe virus dengue, dominansi serotipe

 

Abstract

Background: Although West Java is one of the endemic provinces for DHF in Indonesia, data on the distribution of serotypes in the province is still very limited to specifi c areas within different time periods. In 2000 – 2002, the serotype Den-2 dominated the Bandung city area. The purpose of this study was to identify the distribution of dengue virus serotype in the West Java province and the dominant serotypes in DHF cases.
Methods: Data was collected in October-December 2008 from 27 hospitals in the West Java province. The population for this study was all patients diagnosed with dengue virus hospitalized in the 27 hospitals. Sample was purposively chosen from these patients who were willing to participate in further immunologic examinations. Serum from patients who were immunologically positive was further examined to determine the
dengue virus serotype. This examination was done in the Biomedical and Basic Health Technology Center of the Health Research and Development Unit Republic of Indonesia using the nested RT-PCR method.

Results: Out 513 samples examined, 180 samples could be identifi ed. Dengue virus was not found in the rest of the samples. Four samples showed multiple infections of 2 dengue virus serotypes. All four serotypes were found in the West Java province. In all positive samples, Den-3 was found to be the dominant serotype with 47.8%, followed by Den-2 (26.0%), Den-1 (18.6%), Den-4 (5.4%), and mixed (2.2%). Den-3 was found in all endemic areas of the West Java province.

Conclusion: All 4 dengue virus serotypes can be found in the West Java province with Den-3 serotype the most dominant. (Health Science Indones 2012;1:23-6)

 


Keywords


den-3 serotype; dengue virus serotype distribution; serotype dominance

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Health Science Journal of Indonesia

p-ISSN: 2087-7021
e-EISSN: 2338-3437

Published by : Badan Litbang Kesehatan, Kementerian Kesehatan RI

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