HIGH DOSES OF SOYBEAN, JATI BELANDA AND THEIR COMBINATION EXTRACTS HAVE NO ACUTE TOXIC EFFECTS

Meilinah Hidayat, Sijani Prahastuti, Ellya Rosa Delima, Liasisca Setiawati, Andreanus Andaja Soemardji

Abstract


Latar Belakang: Sebagai terapi antiobesitas kombinasi ekstrak kedelai Detam-1 dan daun Jati Belanda haruslah aman dan bebas dari bahan toksik. Untuk membuktikan keamanan ekstrak tumbuhan obat, diperlukan uji toksisitas akut. Tes ini terdiri dari: nilai LD50, perilaku, berat organ (BO), dan indeks organ (IO) mencit Swiss Webster setelah diberi ekstrak etanol kedelai Detam-1 (EEKD), ekstrak etanol Jati Belanda (EEJB) dan kombinasinya.Metode: Penelitian eksperimental sejati dengan rancangan acak lengkap sesuai ketentuan BPOM tahun 2014 adalah metode penelitian ini. Dua puluh mencit betina Swiss Webster dibagi menjadi empat kelompok perlakuan. Kelompok 1 (kontrol negatif), 2 (EEKD 2.000 mg / kgBB), 3 (EEJB 2.000 mg / kgBB), dan 4 (kombinasi EEKD dan EEJB 1: 2 sebesar 2000 mg / kgBB). Data BO dan IO dianalisis dengan uji t tidak berpasangan dengan α = 0,05.Hasil:  Tidak ada gejala mortalitas dan toksisitas pada semua kelompok, semua mencit berperilaku normal, tidak ada perbedaan bermakna dari BO dan IO dari delapan organ utama semua grup secara statistik (p> 0,05) kecuali paru-paru, hati dan limpa pada kelompok 2. Kesimpulan: Nilai LD50 dari EEKD, EEJB dan kombinasinya seluruhnya di atas 2.000 mg/kgBB, tidak menimbulkan perubahan perilaku mencit; BO dan IO pada mencit yang diberi dosis sangat tinggi EEKD, EEJB dan kombinasinya.

Kata kunci: LD50, perilaku, bobot organ, indeks organ, kedelai Detam-1, daun Jati Belanda

Abstract

Background: As an antiobesity therapy the combination of Detam-1 soybean extract and Jati Belanda leaves extract should be safe and free from toxic material. In order to prove the safety of both medicinal plant’s extract, acute toxicity test is needed. The test consists of : LD50 value, behaviour, organ weight (OW), and organ index (OI) of Webster Swiss mice after feeding with ethanol extract of Detam-1 soybean (EEDS), ethanol extract of Jati Belanda (EEJB) and its combination. The aim of this research was to know the value of Lethal Dose (LD) 50, behavior, organ weight (OW), and organ index (OI) in Swiss Webster mice after administered of ethanol extract of Detam-1 soybean (EEDS), ethanol extract of Jati Belanda (EEJB) and their combination.

Methods: True experimental study with complete randomized design in accordance with BPOM 2014 was the methods of this study. Twenty Swiss Webster female mice were divided into four treatment groups. Group 1 (negative control), 2 EEDS ( 2,000 mg / kgBB), 3 (EEJB 2,000 mg / kgBW), and 4 (EEDS and EEJB 1: 2 combination of 2000 mg / kgBW). OW and OI data were analyzed by independent t test with α = 0.05.

Results: There were no symptom of mortality and toxicity in all groups, all of mice behave normally, statistically no significant differences in OW and OI of the eight major organs of all groups (p> 0.05) except lung, liver and spleen in group 2.

Conclusion: The LD50 value of EEDS, EEJB and their combinations entirely above 2,000 mg/kgBW, no changing on the behavior, OW and OI in mice which given very high dose of EEDS, EEJB and their combination

Keywords : LD50, behavior, organ weight, organ index, Detam-1 soybean, Jati Belanda



Keywords


LD50, behavior, organ weight, organ index, Detam-1 soybean, Jati Belanda

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