Infeksi Ganda Leptospira dan Hantavirus pada Rattus norvegicus di Maumere Flores, Nusa Tenggara Timur

Arief Mulyono, Ristiyanto Ristiyanto, Dimas Bagus Wicaksono Putro

Abstract


ABSTRAK

Kasus leptospirosis dan infeksi Hantavirus dilaporkan mengalami peningkatan berkaitan dengan adanya pemanasan global.  Rattus norvegicus merupakan reservoir dari dua penyakit tersebut. Adanya infeksi ganda Leptospira dan Hantavirus pada R. norvegicus merupakan faktor resiko terjadinya penularan Leptospira dan Hantavirus di populasi manusia. Penelitian ini bertujuan untuk mengetahui prevalensi infeksi ganda Leptospira dan Hantavirus pada R. norvegicus di Kota Maumere, Flores. Jenis penelitian ini adalah penelitian deskriptif dengan desain potong lintang. Penelitian dilakukan pada bulan Agustus sampai November 2014 di daerah perimeter dan buffer pelabuhan Maumere, Flores. Populasi penelitian adalah R. norvegicus di Kota Maumere, Flores. Subyek penelitian adalah R. norvegicus yang tertangkap. Deteksi Leptospira pada R. norvegicus dengan teknik PCR menggunakan primer spesifik untuk gen 16S rRNA dan deteksi Hantavirus pada R. norvegicus dengan teknik nested-PCR menggunakan primer spesifik untuk segmen L. Analisis data dilakukan secara deskriptif.  Hasil penelitian menunjukkan R. norvegicus yang tertangkap sejumlah 114 ekor. Prevalensi Leptospira pada R. norvegicus 4,3%, prevalensi Hantavirus 22,8% dan infeksi ganda Leptospira dan Hantavirus sebesar 1,8%.  Penularan leptospirosis dan infeksi Hantavirus berpotensi terjadinya di Kota Maumere.

Kata kunci:  leptospirosis, Leptospira, Hantavirus, Maumere

ABSTRACT

Infection of Leptospirosis and Hantavirus are increased which related to global warming. Rattus norvegicus is reservoirs of both of diseases infections. Dual infection of Leptospira and Hantavirus in R. norvegicus become risk factor of Leptospira and Hantavirus infection in the human. The objective of this study is to figure out the prevalence of Dual infection in R. norvegicus. This study was descriptive research with cross-sectional design. The population of study was R. norvegicus in Maumere City, Flores. The rats  (n=114) were trapped from perimeter and buffer area of Maumere Port, Flores in August to November 2014. Leptospira in R. norvegicus detected by PCR used specific primers for 16S rRNA gene and Hantavirus by nested – PCR used specific primers for the L segment. The data was analysed using descriptive analysis. The prevalence of Leptospira in R. norvegicus was 4.3%, hantavirus was 22,8% and dual infection of Leptospira and Hantavirus was 1.8%. The transmission dual infection of leptospirosis and Hantavirus are potentially occured in Maumere.

Keywords: leptospirosis, Leptospira, Hantavirus, Maumere



Keywords


leptospirosis, Leptospira, Hantavirus, Maumere

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