Isolasi dan Identifikasi Struktur Karotenoid dari Ekstrak Bayam Merah (Amaranthus Tricolor L.)

Novi Sulistyaningrum

Isolasi dan Identifikasi Struktur Karotenoid dari Ekstrak Bayam Merah (Amaranthus Tricolor L.)

Abstract


Amaranthus tricolor L. was reported as source of carotenoid compounds (tetraterpenoids) which is a natural pigments and has bioactivity as antioxidants. They are beneficial to health. Carotenoids isolate are obtained by maceration method in methanol, saponification with KOH / MeOH 5%, fractionated by vacuum column chromatography, preparative thin layer chromatography (preparative-TLC) repeatedly and each fraction monitored using thin layer chromatography (TLC). Structure analysis of carotenoids based on UV, IR and GC-MS spectroscopic data showed similarities as lutein structure.


Keywords


Amaranthus tricolor L; Carotenoid; Lutein; Spectroscopic

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