PENGEMBANGAN BIAKAN IN-VITRO PLASMODIUM FALCIPARUM SECARA KONTINU

Sekar Tuti, Suwarni Suwarni, Harijani A. M.

PENGEMBANGAN BIAKAN IN-VITRO PLASMODIUM FALCIPARUM SECARA KONTINU

Abstract


To support malaria research on its' serology/immunology, chemotherapy, drug sensitivity aspects etc. especially for falciparum malaria, a large amount of antigen (parasites) is needed. These antigen could not be obtained from patients in the field only.

Considering this situation, attempts have been made to develop a Plasmodium falciparum continuous culture   in-vitro following a method introduced  by Trager and Jensen (1976).

In our laboratory, the parasite grew and multiplied nicely for 60 days. During that period of cultivation, a large amount of parasites (mostly mature trophozoite and schizont stages) have been collected for antigen production. Several tubes of mostly young trophozoites stage have been preserved, it can be cultured again in the future or transported to another laboratory for further culture.


Keywords


Plasmodium Falciparum

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Buletin Penelitian Kesehatan (Bulletin of Health Research, p-ISSN: 0125-9695. e-ISSN: 2338-3453) is published by Badan Penelitian dan Pengembangan Kesehatan, Ministry of Health of Republic of Indonesia
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