AKTIVITAS ENZIM ESTERASE PADA POPULASI NYAMUK AEDES AEGYPTI TERHADAP MALATION DI TIGA KABUPATEN DI PROVINSI DAERAH ISTIMEWA YOGYAKARTA

Sunaryo Sunaryo, Dyah Widiastuti

Abstract


Abstract

Special Region of Yogyakarta is one of the areas with high Dengue Hemorrhagic Fever (DHF) in Indonesia. The use of insecticides for dengue vector control is widely performed in order to control the incidence of DHF. The aim of this research was to know the use of insecticide in controlling Ae.aegypti mosquito, the susceptibility status of Ae aegypti mosquitoes in three districts (Sleman, Gunung Kidul and Bantul) in Yogyakarta against malation and to know the activity of esterase enzyme in the mosquito population. The research was conducted in 2015 with cross sectional design. Ae. aegypti mosquito resistance data to malation was obtained based on the activity of alpha enzyme and beta esterase in mosquito’s body biochemically. Increased activity of esterase enzyme indicates the occurrence of Ae. aegypti resistance against malation insecticides. Data on information on insecticide use were obtained from secondary data from district health offices at the three research sites. The susceptibility test results indicate that an increase in esterase enzyme activity, especially beta esterase in the Ae aegypti population. It can be concluded that in all three research sites there has been Ae aegypti resistance against malation insecticides. This means that the use of malation in the control of Ae. aegypti is no longer effective, resulting in the control of Ae. aegypti in the study sites should select the active ingredient of insecticide which does not have carboxylic acid (malation) group.

 

Keywords: Enzyme esterase, resistance, malation, Aedes aegypti

 

ABSTRAK

Provinsi Daerah Istimewa Yogyakarta (DIY) merupakan salah satu daerah dengan kejadian Demam Berdarah Dengue (DBD) tinggi di Indonesia. Penggunaan insektisida untuk pengendalian vektor DBD secara luas dilakukan dalam rangka mengendalikan kejadian DBD. Tujuan penelitian untuk mengetahui penggunaan insektisida dalam pengendalian nyamuk Ae. aegypti, status kerentanan nyamuk Ae. aegypti di tiga kabupaten (Sleman, Gunung Kidul dan Bantul) di Provinsi DIY terhadap malation serta mengetahui aktivitas enzim esterase pada populasi nyamuk tersebut. Penelitian dilakukan pada tahun 2015 dengan disain cross sectional. Data resistensi nyamuk Ae. aegypti terhadap malation diperoleh berdasarkan aktivitas enzim alfa dan beta esterase pada tubuh nyamuk secara biokimiawi. Meningkatnya aktivitas enzim esterase mengindikasikan telah terjadinya resistensi nyamuk Ae. aegypti terhadap insektisida malation. Data mengenai informasi penggunaan insektisida, diperoleh dari data sekunder dari dinas kesehatan kabupaten pada ketiga lokasi penelitian. Hasil uji kerentanan menunjukkan bahwa adanya peningkatan aktivitas enzim esterase khususnya beta esterase pada populasi nyamuk Ae. aegypti. Dapat disimpulkan bahwa pada ketiga lokasi penelitian telah terjadi resistensi nyamuk Ae. aegypti terhadap insektisida malation. Hal ini berarti bahwa penggunaan malation dalam pengendalian nyamuk Ae. aegypti tidak efektif lagi, sehingga dalam kegiatan pengendalian nyamuk Ae. aegypti di lokasi penelitian sebaiknya memilih bahan aktif insektisida yang tidak memiliki gugus carboxylic acid (malation).

 

Kata kunci: Enzim esterase, resistensi, malation, nyamuk Aedes aegypti


Keywords


enzyme esterase, resistance, malathion, Aedes aegypti

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